National BioResource Project
Last update： April 2, 2020
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Genetic experimental materials, such as plasmid and viral vectors, genomic and cDNA clones, have become one of the most important and fundamental research tools for life sciences. Genetic materials are now widely utilized in numerous fields of life sciences, not only in basic researches such as analyses of gene function and control mechanisms of gene expression but also in applied researches such as development of novel diagnostic and therapeutic methods, drug discovery and material production. The Gene Engineering Division of RIKEN BioResource Center (BRC) has been engaging in the collection, preservation, quality control and distribution of genetic materials developed in Japan by individual scientists and by various national projects.
Recently, genetic materials can be prepared easily by means of, for example, polymerase chain reaction. However, such materials often contain accidental mutations and produce dubious experimental results. To provide domestic and international scientific community with genetic materials of the highest quality and reproducibility, the Gene Engineering Division performs rigorous quality control by testing growth and propagation, restriction enzyme mapping and nucleotide sequencing of clones. Relevant information such characteristics and methodologies is provided via the web site of the RIKEN BRC. For the best use of genetic resources, training courses of advanced technologies are also given.
The Material Transfer Agreement is used for each transfer of genetic materials to protect the intellectual property rights of developers and to define the responsibility of users. We have opened a path of the academic use of genetic materials produced by using advanced research tools owned by commercial entities. Deposition of genetic materials in the RIKEN BRC frees researchers from time consuming preparation and distribution of materials to fellow researchers. Furthermore, deposition increases chance of collaboration and citation of research papers. Your deposition of genetic resources in RIKEN BRC Gene Engineering Division and use of these resources are most appreciated.
- Deposited clones from researchers around the world
Numerous clones developed by individual researchers, large sets of genetic materials from the core facilities of NBRP and other National Projects are available.
[Human full-length cDNAs, cDNAs (mouse, Xenopus laevis, and Ciona intestinalis), BAC clones (mouse, rat, Japanese macaque and Drosophila flies), genomic DNAs (mouse and microbes)]
- Ready for use genetic materials
Users can perform their experiments using our genetic materials directly, without re-constructing vectors. For example, more than 500 recombinant adenoviruses are available for the infection of cultured mammalian cells as well as the mouse.
[Reporter genes (luciferase and GFP), cellulase expression vectors, S. pombe and thermophiles gene plasmid, and recombinant viruses]
Distribution / Deposition
Resource Committee of Genetic Materials
Resource Committee of Information
Recombinant Virus Hub
Panel exhibition at the The 32nd Annual Meeting of the Molecular Biology Society of Japan.