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NBRP Genome Information Upgrading Program Project's Progress


Focus Genome Re-sequencing of Diverse Strains of Bombyx mori and B. mandarina
Principal Investigator Toru Shimada   The University of Tokyo
Project Period FY2015
Overview   In the Silkworm NBRP, 500 or more mutant strains and geographical races are maintained and distributed to many researchers. The silkworm genome information was accurately assembled and published in 2008 by collaboration between Japanese and Chinese groups. After that, many researchers have actively carried out the analysis of gene function, especially positional cloning of the genes responsible for the phenotypes by utilizing the genomic information. But, the genome sequences of the Silkworm NBRP strains have not yet been analyzed, and many users must find the DNA markers necessary for linkage analysis by themselves. In this program, we will perform the re-sequencings of 18 strains of the domestic silkworm Bombyx mori and 2 strains of the wild species B. mandarina by the next-generation sequencer. These 20 strains have been carefully selected to represent the genetic diversity of the silkworms. Some of them have been maintained by inbreeding for about 100 years, and are expected to be highly homozygous. The resultant data will be registered in DDBJ, and publicized at NBRP SilkwormBase and SilkBase. These 20 strains have big varieties in their morphology, coloration, development, diapause, and cocoon/silk traits. Genomic information of these strains will dramatically upgrade the value of the Silkworm NBRP resources.
  This program is performed in close collaboration between the University of Tokyo (T. Shimada and T. Kiuchi) and the National Institute of Genetics (A. Toyoda).

Project's Progress B. mori (c10), B. mori (p21), B. mori (p22), B. mori (p20), B. mori (k25),
B. mori (f35), B. mori (b20), B. mori (d18), B. mori (u48), B. mori (n16),
B. mori (o55), B. mori (o56), B. mori (e10), B. mori (p44), B. mori (c51),
B. mori (g53),B. mandarina (Oki), B. mandarina (Sakado), B. mori (C108T),
B. mori (N4)