|Focus||Generation of genome sequence diversity information for wild relatives of rice|
|Principal Investigator||Nori Kurata Genetic Resource Center, National Institute of Genetics|
The NBRP rice resource project has been making progress on the issue of “Construction of rice resource foundation for harnessing the diversity of the genus Oryza”.
In the program we have prepared about 1700 accessions of wild relatives of rice from 9 genome types and 23 species covering almost all of the wild species in the genus Oryza, originally collected from all over the world.
Among them, we selected 280 accessions as a core collection and have investigated their various trait characters.
We have accumulated genome information for an additional 350 wild AA genome accessions at about 10% genome sequence coverage.
An international consortium for comparative genomics of rice is now performing genome-wide sequencing on wild diploid species such as those possessing AA, BB, CC, EE, FF and GG genomes.
As part of that consortium, we, the National Institute of Genetics group, were assigned to construct CC genome sequences and have been making progress on doing so for one of the species.
As part of the proposed program, we plan to construct precise pseudo-molecules for the remaining two CC genome species. In addition, we plan to read the sequences of about 300 representative accessions for all genome types at x10 depth and aiming to achieve more than 50% coverage of each genome. This high quality data would serve as an indicator for a molecular basis of genome diversity for each accession and species. This information will be released to researchers worldwide to form a foundation for diversity and evolutionary studies of Oryza. We believe that this information together with our biological resources will contribute to future progress in the research community.