|Focus||Targeted genome resequencing of 20 strains of the rats|
|Principal Investigator||Mikita Suyama Medical Institute of Bioregulation, Kyushu University|
Rats have been used as animal models of human diseases such as cancer and hypertension. NBRP-Rat is the largest repository for the rat strains, and currently about 700 strains are registered in the repository. Among those strains, there are many rat models of immune-mediated disorders and cancers.
This project focuses on target capture resequencing of 20 strains of the rats, which include four strains of the representative inbred rats, seven strains of the inbred rats originated from the wild populations, and nine strains of disease models derived from selective breeding. The target regions used in the project cover highly conserved genomic regions among vertebrates as well as all the exonic regions. With this method, we can detect mutations not only in coding regions but also in regulatory regions such as those in enhancers in highly efficient manner. The project is conducted in the collaboration with The Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, which is the NBRP Center for rat strains, and Kazusa DNA Research Institute. Through the target capture sequencing of the rats, we collect and open the data of polymorphisms in these strains to the public to enhance the activities of the research community of rats.
F344/DuCrlCrlj, F344/Stm, F344/NSlc, F344/Jcl, IS/Kyo, IS-Tlk/Kyo, LEC/Tj, NIG-III/Hok, LE/Stm, DOB/Oda, BN/SsNSlc, PVG/Seac, BUF/MNa, HTX/Kyo, BDIX.Cg-Tal/NemOda, BDIX/NemOda, KFRS3B/Kyo, HWY/Slc, ZFDM, ZF