|Focus||Genome resequencing of Japanese wild mouse-derived MSM/Ms strain|
|Principal Investigator||Toyoyuki Takada National Institute of Genetics|
The laboratory mouse is one of the most important bioresources for studying principles underlying higher-order biological
phenomena and etiologies of human diseases. National Institute of Genetics (NIG) has established a series of mouse experimental
strains named “Mishima Battery” since 1970’s. It consists of ten inbred strains originated from three or four subspecies of
Mus musculus, which are widely distributed in the world. These strains have very remote genetic status from the commonly used
laboratory mouse strains such as C57BL/6J (B6), and show very unique phenotypes. The mouse strains in “Mishima Battery”
are now distributed to the science research community via RIKEN BioResource center, and contribute to broad range of life
science. NIG also established B6-ChrNMSM consomic strains, in which every chromosome of B6 is substituted by corresponding
chromosome of Japanese wild mouse-derived MSM/Ms (MSM) strain, which is a member of “Mishima Battery”.
For genome analysis of MSM, we previously performed a resequencing project of MSM, which was supported by “NBRP genome information upgrading program” and KAKENHI on Priority Areas “Comparative Genomics”. Our study revealed more than ten millions of nucleotide substitutions between B6 reference genome and the MSM genome. In this program, to elucidate more in detail of structure and copy number variation, further resequencing by single molecule and real-time sequencing system is employed for the MSM genome as collaboration with Comparative Genomics Laboratory, and Advanced Genomics Center, NIG, and Joint Support-Center for Data Science Research, ROIS. Upcoming resequenced data will be open to public to increase usability of the MSM genome information for the research community via NIG mouse genome database “NIG_MoG” (http://molossinus.nig.ac.jp/msmdb/index.jsp).